Composite

Part:BBa_K4905011:Design

Designed by: Merel van den Bosch   Group: iGEM23_TU-Eindhoven   (2023-08-15)


Elastin-Like Polypeptide Triblock with FKBP12


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2222
    Illegal XbaI site found at 339
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2222
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2222
    Illegal XhoI site found at 2239
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2222
    Illegal XbaI site found at 339
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2222
    Illegal XbaI site found at 339
    Illegal NgoMIV site found at 396
    Illegal NgoMIV site found at 576
    Illegal NgoMIV site found at 666
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 3168
    Illegal NgoMIV site found at 3345
    Illegal NgoMIV site found at 3435
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

ELPs are repetitive sequences that are not suitable for PCR. Colony PCR is needed to obtain more of the DNA from this part.


Source

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6611466/#:~:text=FKBP12%20is%20a%2012%20kDa,folding%20%5B1%2C%202%5D.

References